gene expression quantification of toll like receptors 2, 4 and co-molecules in human glioblastoma cell line (u87-mg): toward a new in vitro model of inflammation

نویسندگان

soliman mohammadi-samani department of pharmaceutics, school of pharmacy, shiraz university of medical sciences

gholamhossein yousefi department of pharmaceutics, school of pharmacy, shiraz university of medical sciences

farhad mohammadi department of pharmaceutics, school of pharmacy, shiraz university of medical sciences

fatemeh ahmadi department of pharmaceutics, school of pharmacy, shiraz university of medical sciences

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Gene Expression Quantification of Toll like Receptors 2, 4 and Co-molecules in Human Glioblastoma Cell Line (U87-MG): Toward a New In vitro Model of Inflammation

OBJECTIVES Pattern recognition receptors (PRRs) are the main part in the innate immune response. Human glioblastoma cell line (U87-MG) is an established adherent cell line model of this common cancer; due to genetic variations between individuals it is likely more suitable for investigating molecular aspects of innate immunity. Therefore, we undertook a novel characterization of the immune phen...

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Down-Regulation of CD14 Transcripts in Human Glioblastoma Cell Line U87 MG

Background: Pattern recognition receptors (PRRs) are the main sensors of pathogen and danger signals in innate immunity of which Toll Like Receptors (TLRs) are the most studied ones. The contribution of PRRs in cerebral inflammation induced by microbial infection, tissue damage and cancer has not extensively been addressed so far. Glioma is the most common tumor of the central nervous system an...

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The effect of Citrullus colocynthis on the induction of Casp3 and Casp8 gene expression on human Glioblastoma U87 cell line

Introduction: Using in vitro MTT assay and Real-time PCR, the cytotoxicity and cell inhibition mechanism of using Citrullus colocynthis on human glioblastoma U87 cell line was investigated. Methods: The plant extract was extracted using ethanol (70%). To investigate the cytotoxic effects of Citrullus colocynthis extract on U87 cell line, 10 4 cells per well was incubated with different concentr...

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P-17: Expression of Cell Surface Toll-Like Receptors in the Human Male Reproductive Tract

Background: Male infertility refers to the inability of a male to achieve a pregnancy in a fertile female. The root of many causes of infertility is miscommunication between immune and reproductive system. Male reproductive system is very sensitive and vulnerable, infections can hinder maturation and movement of spermatozoa lead to impaired fertility.All species need an immediate reply to the m...

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عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۷، شماره ۷، صفحات ۵۴۶-۵۴۶

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